DNA aptamers targeting P. aeruginosa RNAP

Abstract

We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandem purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.